Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi

Aline Lourdes Martins Silva; Marcos Diones Ferreira Santana; John César de Jesus Pereira; Milena Pupo Raimam; Ulisses Brigatto Albino

doi:10.4336/2015.pfb.35.84.798

Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi

Authors

Aline Lourdes Martins Silva Universidade Federal do Pará, Faculdade Ciências Biológicas
Marcos Diones Ferreira Santana Universidade Federal do Oeste do Pará
John César de Jesus Pereira Universidade Federal do Pará
Milena Pupo Raimam Universidade do Estado do Pará
Ulisses Brigatto Albino Universidade Federal do Pará, Faculdade Ciências Biológicas

DOI:

https://doi.org/10.4336/2015.pfb.35.84.798

Keywords:

Natural dyes, Soil microbiology, Euterpe oleracea

Abstract

Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.

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Author Biographies

Aline Lourdes Martins Silva, Universidade Federal do Pará, Faculdade Ciências Biológicas

http://lattes.cnpq.br/0166337764589744

Marcos Diones Ferreira Santana, Universidade Federal do Oeste do Pará

http://lattes.cnpq.br/9439828625006426

John César de Jesus Pereira, Universidade Federal do Pará

http://lattes.cnpq.br/3554448134797033

Milena Pupo Raimam, Universidade do Estado do Pará

http://lattes.cnpq.br/0300891230891721

Ulisses Brigatto Albino, Universidade Federal do Pará, Faculdade Ciências Biológicas

http://lattes.cnpq.br/1485924743638488

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Published

2015-12-31

How to Cite

SILVA, Aline Lourdes Martins; SANTANA, Marcos Diones Ferreira; PEREIRA, John César de Jesus; RAIMAM, Milena Pupo; ALBINO, Ulisses Brigatto. Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi. Pesquisa Florestal Brasileira, [S. l.], v. 35, n. 84, p. 475–479, 2015. DOI: 10.4336/2015.pfb.35.84.798. Disponível em: https://pfb.sede.embrapa.br/pfb/article/view/798. Acesso em: 24 apr. 2026.

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Issue

Vol. 35 No. 84 (2015): out./dez.

Section

Scientific Notes

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