Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
DOI:
https://doi.org/10.4336/2015.pfb.35.84.798Keywords:
Natural dyes, Soil microbiology, Euterpe oleraceaAbstract
Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2015 Aline Lourdes Martins Silva, Marcos Diones Ferreira Santana, John César de Jesus Pereira, Milena Pupo Raimam, Ulisses Brigatto Albino
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
PFB reserves the right to edit manuscripts to correct grammar/spelling, improve clarity, and comply with the journal’s standards while maintaining the style of the authors.
The final version will be sent to the corresponding author for approval.
Published articles become the property of PFB.
Manuscripts may be used after publication without prior authorization from PFB, as long as the journal is credited.
Warning: figures published in PFB may only be reused with prior authorization from Embrapa Forestry.
All content in PFB is licensed under Creative Commons attribution (type BY-NC-ND).
The opinions and concepts expressed in manuscripts are the sole responsibility of their respective authors and not PFB.
Os originais publicados na Pesquisa Florestal Brasileira estão disponibilizados de acordo com uma Licença 