Callus induction in vitro on leaves explants of Ilex paraguariensis

Authors

  • Thamires Weigert Stachevski Pontíficia Universidade Católica do Paraná - PUC/PR
  • Luziane Franciscon Embrapa Florestas
  • Juliana Degenhardt-Goldbach Embrapa Florestas

DOI:

https://doi.org/10.4336/2013.pfb.33.75.441

Keywords:

Zeatin, 2, 4-D, Rizogenesis, Organogenesis

Abstract

Organogenesis is a technique rarely studied in the micro propagation of Ilex paraguariensis. This study evaluated different culture media on callus induction and in vitro organogenesis of this species. Leaves were collected from greenhouse grown plants. Leaves segments were placed on culture media ¼ MS, WPM or JADS, containing zeatin and 2,4-D. The MS medium was more efficient in callus inducing. Rooting was observed in the WPM medium. No buds were formed in any of the media evaluated.

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Author Biographies

  • Thamires Weigert Stachevski, Pontíficia Universidade Católica do Paraná - PUC/PR
    Graduanda em Biologia
  • Luziane Franciscon, Embrapa Florestas
    Possui graduação em Estatística pela Universidade Federal do Paraná (2005). Mestre em Estatística e Experimentação Agronômica pela ESALQ/USP (2008). Tem experiência na área de Estatística, atuando principalmente nos seguintes temas: estatística experimental, espacial e modelos de regressão, desenvolve projetos junto ao LEG/UFPR (Laboratório de Estatística e Geoinformação).
  • Juliana Degenhardt-Goldbach, Embrapa Florestas
    Possui graduação em Agronomia pela Universidade Federal de Santa Catarina (1999), mestrado em Recursos Genéticos Vegetais pela Universidade Federal de Santa Catarina (2001) e doutorado em Ciências da Horticultura - Leibniz Universidade de Hannover , Alemanha (2006).

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Published

2013-09-30

Issue

Vol. 33 No. 75 (2013): jul./set.

Section

Scientific Notes

License

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How to Cite

STACHEVSKI, T. W.; FRANCISCON, L.; DEGENHARDT-GOLDBACH, J. Callus induction in vitro on leaves explants of Ilex paraguariensis. Pesquisa Florestal Brasileira, v. 33, n. 75, p. 339–342, 30 Sep.2013.

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