Individual genomic DNA isolation of Entomogenous nematodes: the Deladenus study case

Authors

  • Guilherme Schnell e Schuhli Embrapa Florestas
  • Susete do Rocio Chiarello Penteado Embrapa Florestas
  • Daiane Rigoni Kestring Embrapa Florestas
  • Gisleine Jarenko Steil Universidade Federal do Paraná, Setor de Ciências Biológicas
  • Micheli Cristina Thomas Universidade Federal do Paraná, Setor de Ciências Biológicas

DOI:

https://doi.org/10.4336/2012.pfb.32.72.483

Keywords:

DNA extraction, Protocol, Woodwasp, Sirex Noctilio

Abstract

Populational studies involve single individual DNA extraction in order to grant further genotyping data. In the case of some parasitic nematodes, the reduced dimensions and high individual number per infestation makes individual genotyping a difficult task. Aiming the development of a protocol we performed adjustments in available methods in order to acquire the best gain in purity and concentration of genomic DNA. Single specimens were digested in Worm Lysis Buffer and submitted to PCR amplification as a concept test. It was possible to obtain good amount and concentration of DNA from individuals. Quality was sufficient to grant subsequent ITS1 sequencing.

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Published

2012-12-17

How to Cite

SCHUHLI, Guilherme Schnell e; PENTEADO, Susete do Rocio Chiarello; KESTRING, Daiane Rigoni; STEIL, Gisleine Jarenko; THOMAS, Micheli Cristina. Individual genomic DNA isolation of Entomogenous nematodes: the Deladenus study case. Pesquisa Florestal Brasileira, [S. l.], v. 32, n. 72, p. 483, 2012. DOI: 10.4336/2012.pfb.32.72.483. Disponível em: https://pfb.sede.embrapa.br/pfb/article/view/396. Acesso em: 19 apr. 2025.

Issue

Vol. 32 No. 72 (2012): out./dez.

Section

Scientific Notes

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