Disinfection and in vitro establishment of Acacia mearnsii nodal segments
DOI:
https://doi.org/10.4336/2017.pfb.37.92.1392Keywords:
Black wattle, Clones, CuttingAbstract
Due to low rates of rooting it is difficult to propagate selected clones of Acacia mearnsii by cuttings. The purpose of this study was to define a protocol for disinfection of nodal segments for in vitro introduction, aiming to improve the species genetically for forest plantation. Combination of different products concentration (70% ethyl alcohol, sodium hypochlorite, plant preservative mixture and mercuric chloride), immersion period and size of nodal segment were tested. The best disinfection treatment was the combination of 70% ethanol, 0.4% mercuric chloride, sodium hypochlorite at 2% in nodal segments of 10 mm, with 80% survival.
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References
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Correia, D. & Graça, M. E. C. In vitro propagation of Black wattle (Acacia mearnsii De Wild.). IPEF, n. 48/49, p. 117-125, 1995.
Davey, M. R. & Anthony, P. Plant cell culture: essential methods. Chichester, UK: Willey-Blackwell, 2010. 359 p.
Dutra, L. F. et al. A micropropagação de Eucalipto. Pesquisa Florestal Brasileira, n. 58, p. 49-59, 2009. DOI: 10.4336/2009.pfb.58.49.
Dutra, L. F. et al. Introdução ao cultivo in vitro de Erva-mate (Ilex paraguariensis). Colombo: Embrapa Florestas, 2008. (Embrapa Florestas. Boletim de pesquisa e desenvolvimento, 38).
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Flores, R. et al. Otimização da micropropagação de Pfaffia tuberosa (Spreng.) Hicken. Revista Ciência Rural, v. 36, n. 3, p. 845-851, 2006.
Golle, D. P. et al. Desinfestação superfici.al de explantes isolados de ramos semilenhosos e herbáceos de Eugenia involucrata DC. (Myrtaceae). Revista Cerne, v. 19, n. 1, p. 77-82, 2013.
Grattapaglia, D. & Machado, M, A. Micropropagação. In: Torres, A. C. et al. Cultura de tecidos e transformação genética de plantas. Brasília, DF: Embrapa, 1998. p. 183-260.
Ling, W. & Yan, X. Research on tissue culture and propagation of Populus deltoids cl."725". Journal of Anhui Agricultural University, v. 40, p. 612-617, 2013.
Murashige, T. & Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, v. 15, p. 473-497, 1962.
Oliveira, L. S. de et al. Micropropagação de espécies florestais brasileiras. Pesquisa Florestal Brasileira, v. 33, n. 76, p. 439-453, 2013. DOI: 10.4336/2013.pfb.33.76.481.
Ribas, L. L. F. et al. Estabelecimento de culturas assépticas de Aspidosperma polyneuron. Revista Ciência Florestal, v. 13, n. 1, p. 115-122, 2003.
Taiz, L. & Zeiger, E. Fisiologia vegetal. 5. ed. Porto Alegre: Artmed, 2013. 918 p.
Xavier, A. & Otoni, W. C. Aplicações da micropropagação na clonagem de Eucalyptus no Brasil. Agronomía Costarricense, v. 33, n. 2, p. 303-307, 2009.
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Published
2017-12-29
How to Cite
SALLES, Ecléia Alexandra Poltronieri Buda; ALCANTARA, Giovana Bomfim; QUOIRIN, Marguerite Germaine Ghislaine; GONÇALVES, Antonio Natal; HIGA, Antonio Rioyei. Disinfection and in vitro establishment of Acacia mearnsii nodal segments. Pesquisa Florestal Brasileira, [S. l.], v. 37, n. 92, p. 485–491, 2017. DOI: 10.4336/2017.pfb.37.92.1392. Disponível em: https://pfb.sede.embrapa.br/pfb/article/view/1392. Acesso em: 12 dec. 2025.
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