Propagation of farinha-seca in vitro

Abstract

The present study aimed to establish protocols for disinfestation, germination of seeds, as well as the induction of in vitro multi sprouting of farinha-seca (Albizia niopoides) using seeds and nodal segments from seedlings germinated in vitro. To evaluate the disinfestations and germination intervals of 0,10, 20 and 30 minutes of soaking seeds in sodium hypochlorite 8% were tested. The evaluations of contamination by fungi and/or bacteria, as well as the germination of seeds were performed 20 days after the test started. The induction of multi sprouting was performed in WPM culture medium supplemented with BAP at concentration of 0.0, 0.5, 2.5 and 5.0 μM and ANA at a concentration of 0.5 μM. Number of sprouts and callus formation were evaluated. The F test did not revealed significant difference, in the percentages of disinfection and germination, considering the intervals of soaking in sodium hypochlorite. The percentage of disinfestation ranged from 93% to 97% and germination of 67% to 73%. The highest rate of regeneration of axillary sprout (2.6) was obtained with the combination of 5.0 μM of BAP + ANA 0.5 μM, 30 days after inoculation. It was also noted that without the addition of growth regulators in WPM medium, seedlings of Albizia niopoides achieved good rates of sprouts (2.3 sprouts).