Enraizamento in vitro e aclimatização de plântulas de Corymbia citriodora

Luiz Filipe  Maravilha; Miranda Titon; Vitória de Souza Canguçu; Fabiana Miranda Rocha; Marcio Leles Romarco de Oliveira

doi:10.4336/2023.pfb.43e202102232

Authors

Luiz Filipe Maravilha Universidade Federal dos Vales do Jequitinhonha e Mucuri https://orcid.org/0000-0001-5846-3516
Miranda Titon Universidade Federal dos Vales do Jequitinhonha e Mucuri https://orcid.org/0000-0002-5940-5390
Vitória de Souza Canguçu Universidade Federal dos Vales do Jequitinhonha e Mucuri https://orcid.org/0000-0002-5052-2363
Fabiana Miranda Rocha Universidade Federal dos Vales do Jequitinhonha e Mucuri https://orcid.org/0000-0002-0845-466X
Marcio Leles Romarco de Oliveira Universidade Federal dos Vales do Jequitinhonha e Mucuri https://orcid.org/0000-0002-8097-1135

DOI:

https://doi.org/10.4336/2023.pfb.43e202102232

Keywords:

Tissue culture, Indolebutyric acid, Seal

Abstract

The objective of this study was to evaluate the effect of indolbutyric acid (IBA) concentrations on in vitro rooting of Corymbia citriodora juvenile shoots, and sealing forms on the acclimatization of micropropagated seedlings. For rooting, shoots were placed in a culture medium with four concentrations of IBA (0, 1.0, 2.0, and 4.0 mg L^-1). In pre-acclimatization, seedlings germinated in vitro and without contamination were transplanted into polyethylene cups containing substrate. The cups were packed in plastic bags for 10 days and kept in a culture room. Subsequently, three treatments were applied: no coverage; intact plastic bag, and perforated plastic bag. In acclimatization, the history of pre-acclimatization treatments was maintained. The seedlings were transplanted into tubes containing substrate and kept initially in a greenhouse and then in a shade house. The results demonstrate that concentrations of 1.0; 2.0 and 4.0 mg L^-1 of IBA were efficient in the rooting of juvenile explants of C. citriodora, and the highest concentration used provided the highest average number of roots. The absence of cover in pre-acclimatization is recommended for greater survival rate, height, and dry mass of seedlings in the acclimatization stage.

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Author Biographies

Luiz Filipe Maravilha, Universidade Federal dos Vales do Jequitinhonha e Mucuri

http://lattes.cnpq.br/2433077687763878

Miranda Titon, Universidade Federal dos Vales do Jequitinhonha e Mucuri

http://lattes.cnpq.br/3881685523998904

Vitória de Souza Canguçu, Universidade Federal dos Vales do Jequitinhonha e Mucuri

http://lattes.cnpq.br/1220607376231114

Fabiana Miranda Rocha, Universidade Federal dos Vales do Jequitinhonha e Mucuri

http://lattes.cnpq.br/3406574741544730

Marcio Leles Romarco de Oliveira, Universidade Federal dos Vales do Jequitinhonha e Mucuri

http://lattes.cnpq.br/1808132114787261

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