Growth regulators on callogenesis and rhizogenesis in Eugenia involucrata
DOI:
https://doi.org/10.4336/2020.pfb.40e201901908Keywords:
Plant biotechnology, Organogenesis, Vegetative propagationAbstract
This study evaluated the effect of growth regulators on callogenesis and rhizogenesis in Eugenia involucrata. In two experiments, we tested the naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D) and BAP associations. After 45 days of culture, all treatments formed calli, however, those treatments with NAA (alone or combined with BAP) and 2,4-D combined with BAP (5:5 and 10:10 μM) presented the best results. Only the use of NAA at 10 μM induced rhizogenesis. At 90 days of culture, the effect of phytoregulators was most evident, where 2,4-D combined with BAP (5:5 and 10:10 μM) were more favorable for callogenesis while 10 μM NAA were more favorable for rhizogenesis. These results indicate that cell dedifferentiation and re-differentiation processes are achievable from leaf explants of Eugenia involucrata.Downloads
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References
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Murashige, T. & Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v. 15, n. 3, p. 473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.
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Pinto, D. L. P. et al. Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes. Plant Cell, Tissue and Organ Culture, v. 107, n. 3, p. 521-530, 2011. https://doi.org/10.1007/s11240-011-0003-y.
Rocha, D. I. & Dornelas, M. C. Alternative induction of de novo shoot organogenesis or somatic embryogenesis from in vitro cultures of mature zygotic embryos of passion fruit (Passiflora edulis Sims) is modulated by the ratio between auxin and cytokinin in the medium. Plant Cell, Tissue and Organ Culture, v. 120, n. 3, p. 1087-1098, 2015. https://doi.org/10.1007/s11240-014-0663-5.
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Rossato, M. et al. Multiplication and in vitro rooting of Campomanesia adamantium Camb. Plant Cell Culture & Micropropagation, v. 11, n. 2, p. 70-77, 2015.
Santos, B. R. et al. Indução de calos friáveis em explantes foliares de salix (Salyx humboldtiana Willd). Ciência Rural, v. 35, n. 3, p. 510-514, 2005. http://dx.doi.org/10.1590/S0103-84782005000300004.
Shen, X. et al. Assessment of somaclonal variation in Dieffenbachia plants regenerated through indirect shoot organogenesis. Plant Cell, Tissue and Organ Culture, v. 91, n. 1, p. 21-27, 2007. https://doi.org/10.1007/s11240-007-9273-9.
Silva, M. L. et al. A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos. Plant Cell, Tissue and Organ Culture, v. 99, n. 1, p. 47-54, 2009. https://doi.org/10.1007/s11240-009-9574-2.
Sorin, C. et al. Auxin and light control of adventitious rooting in Arabidopsis require Argonaute. The Plant Cell, v. 17, n. 5, p. 1343 - 1359, 2005. https://doi.org/10.1105/tpc.105.031625.
Stachevski, T. W. et al. Efeito do meio de cultura na calogênese in vitro a partir de folhas de erva-mate. Pesquisa Florestal Brasileira, v. 33, n. 75, p. 339-342, 2013. https://doi.org/10.4336/2013.pfb.33.75.441.
Taiz, L. et al. Fisiologia e desenvolvimento vegetal. 6. ed. Porto Alegre: Artmed, 2017. 888 p.
Termignoni, R. R. Cultura de tecidos vegetais. Porto Alegre: Ed da UFRGS. 2005. 182 p.
Webster, S. A. et al. Somatic embryogenesis from leaf and zygotic embryo explants of Blighia sapida "cheese" ackee. In Vitro Cellular & Developmental Biology - Plant, v. 42, n. 5, p. 467-472, 2006. https://doi.org/10.1079/IVP2006795.
Werner, E. T. et al. Meios de cultura, reguladores de crescimento e fontes de nitrogênio na regulação da calogênese do pau-brasil (Caesalpinia echinata Lam.). Acta Botânica Brasileira, v. 24, n. 4, p. 1046-1051, 2010. http://dx.doi.org/10.1590/S0102-33062010000400019.
Aragão, A. K. O. et al. O efeito do BAP (6-benzilaminopurina) sobre a indução de brotos em explantes de pau-brasil. Cerne, v. 17, n. 3, p. 339-345, 2011. http://dx.doi.org/10.1590/S0104-77602011000300007.
Backes, P. & Irgang, B. Árvores do Sul: guia de identificação e interesse ecológico. Porto Alegre: Paisagem do Sul, 2009. 325 p.
Baque, M. A. et al. Induction mechanism of adventitious root from leaf explants of Morinda citrifolia as affected by auxin and light quality. In vitro Cellular and Developmental Biology - Plant, v. 46, n. 1, p. 71-80, 2010. https://doi.org/10.1007/s11627-009-9261-3.
Carneiro, F. S. et al. Embriogênese somática em Agave sisalana Perrine: indução, caracterização anatômica e regeneração. Pesquisa Agropecuária Tropical, v. 44, n. 3, p. 294-303, 2014. http://dx.doi.org/10.1590/S1983-40632014000300005.
Carvalho, M. A. F. et al. Indução, análises morfológicas e ultraestruturais de calos de maracujazeiro nativo. Ceres, v. 62, n. 4, p. 340-346, 2015. http://dx.doi.org/10.1590/0034-737X201562040002.
Carvalho, P. E. R. (Ed.). Espécies arbóreas brasileiras. Brasília, DF: Embrapa Informação Tecnológica; Colombo: Embrapa Florestas, 2014. 634 p. (Coleção espécies arbóreas brasileiras, v. 5).
Ferreira, D. F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, v. 35, n. 6, p. 1039-1042, 2011. http://dx.doi.org/10.1590/S1413-70542011000600001.
Fett-Neto, A. G. et al. Distinct effects of auxin and light on adventitious root development in Eucalyptus saligna and Eucalyptus globulus. Tree Physiology, v. 21, n. 7, p. 457- 464, 2001. http://dx.doi.org/10.1093/treephys/21.7.457.
Grattapaglia, D. & Machado, M. A. Micropropagação. In: Torres, A. C. et al. Cultura de tecidos e transformação genética de plantas. Brasília, DF: Embrapa-SPI: Embrapa-CNPH, 1998. p. 183-260.
Lorenzi, H. (Ed). Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. 3. ed. Nova Odessa: Instituto Plantarum, 2009. 384 p. v. 2.
Ma, X. Y. et al. Leaf callus induction and suspension culture establishment in lychee (Litchi chinensis Sonn.) cv. Huaizhi. Acta Physiologiae Plantarum, v. 31, n. 2, p. 401-405, 2009. http://dx.doi.org/10.1007/s11738-008-0223-x.
Morais, T. P. et al. Reguladores de crescimento vegetal no cultivo in vitro de Mentha x Piperita L. Revista Brasileira de Plantas Medicinais, v. 16, n. 2, supl. I, p. 350-355, 2014. http://dx.doi.org/10.1590/1983-084X/13_017.
Murashige, T. & Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v. 15, n. 3, p. 473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.
Pacurar, D. I. et al. Auxin is a central player in the hormone cross-talks that control adventitious rooting. Physiologia Plantarum, v. 151, n. 1, p. 83-96, 2014. https://doi.org/10.1111/ppl.12171.
Pasquali, G. & Zanettini, M. H. B. Transgênese florestal. In: BORÉM, A. Biotecnologia florestal. Viçosa, MG: Ed. UFV, 2007. p. 317-334.
Pereira, D. M. S. et al. Induction and morphological and biochemical characterization of Hyptis leucocephala (Lamiaceae) calluses. Sitientibus série Ciências Biológicas, v. 12, n. 1, p. 151-156, 2012. https://doi.org/10.13102/scb124.
Pinto, D. L. P. et al. Ploidy stability of somatic embryogenesis-derived Passiflora cincinnata Mast. plants as assessed by flow cytometry. Plant Cell, Tissue and Organ Culture, v. 103, n. 1, p. 71-79, 2010. https://doi.org/10.1007/s11240-010-9756-y.
Pinto, D. L. P. et al. Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes. Plant Cell, Tissue and Organ Culture, v. 107, n. 3, p. 521-530, 2011. https://doi.org/10.1007/s11240-011-0003-y.
Rocha, D. I. & Dornelas, M. C. Alternative induction of de novo shoot organogenesis or somatic embryogenesis from in vitro cultures of mature zygotic embryos of passion fruit (Passiflora edulis Sims) is modulated by the ratio between auxin and cytokinin in the medium. Plant Cell, Tissue and Organ Culture, v. 120, n. 3, p. 1087-1098, 2015. https://doi.org/10.1007/s11240-014-0663-5.
Rosado, L. D. S. et al. Aspectos do cultivo in vitro do manjericão cv. Maria Bonita (Ocimum basilicum L.). Plant Cell Culture & Micropropagation, v. 5, n. 2, p. 71-78, 2009.
Rossato, M. et al. Multiplication and in vitro rooting of Campomanesia adamantium Camb. Plant Cell Culture & Micropropagation, v. 11, n. 2, p. 70-77, 2015.
Santos, B. R. et al. Indução de calos friáveis em explantes foliares de salix (Salyx humboldtiana Willd). Ciência Rural, v. 35, n. 3, p. 510-514, 2005. http://dx.doi.org/10.1590/S0103-84782005000300004.
Shen, X. et al. Assessment of somaclonal variation in Dieffenbachia plants regenerated through indirect shoot organogenesis. Plant Cell, Tissue and Organ Culture, v. 91, n. 1, p. 21-27, 2007. https://doi.org/10.1007/s11240-007-9273-9.
Silva, M. L. et al. A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos. Plant Cell, Tissue and Organ Culture, v. 99, n. 1, p. 47-54, 2009. https://doi.org/10.1007/s11240-009-9574-2.
Sorin, C. et al. Auxin and light control of adventitious rooting in Arabidopsis require Argonaute. The Plant Cell, v. 17, n. 5, p. 1343 - 1359, 2005. https://doi.org/10.1105/tpc.105.031625.
Stachevski, T. W. et al. Efeito do meio de cultura na calogênese in vitro a partir de folhas de erva-mate. Pesquisa Florestal Brasileira, v. 33, n. 75, p. 339-342, 2013. https://doi.org/10.4336/2013.pfb.33.75.441.
Taiz, L. et al. Fisiologia e desenvolvimento vegetal. 6. ed. Porto Alegre: Artmed, 2017. 888 p.
Termignoni, R. R. Cultura de tecidos vegetais. Porto Alegre: Ed da UFRGS. 2005. 182 p.
Webster, S. A. et al. Somatic embryogenesis from leaf and zygotic embryo explants of Blighia sapida "cheese" ackee. In Vitro Cellular & Developmental Biology - Plant, v. 42, n. 5, p. 467-472, 2006. https://doi.org/10.1079/IVP2006795.
Werner, E. T. et al. Meios de cultura, reguladores de crescimento e fontes de nitrogênio na regulação da calogênese do pau-brasil (Caesalpinia echinata Lam.). Acta Botânica Brasileira, v. 24, n. 4, p. 1046-1051, 2010. http://dx.doi.org/10.1590/S0102-33062010000400019.
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2020-09-30
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GOLLE, Diego Pascoal; REINIGER, Lia Rejane Silveira; STEFANEL, Charlene Moro; SERROTE, Caetano Miguel Lemos. Growth regulators on callogenesis and rhizogenesis in Eugenia involucrata. Pesquisa Florestal Brasileira, [S. l.], v. 40, 2020. DOI: 10.4336/2020.pfb.40e201901908. Disponível em: https://pfb.sede.embrapa.br/pfb/article/view/1908. Acesso em: 7 jun. 2025.
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