DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations

Authors

  • Ahmad Abdul Latif Hamze Unesp
  • Antonio Nascim Kalil Filho Embrapa Florestas
  • Valderês Aparecida de Sousa Embrapa Florestas
  • Álvaro Figueredo dos Santos Embrapa Florestas
  • Edinelson José Maciel Neves Embrapa Florestas

Keywords:

Heart-palm, Arecaceae, Protocol

Abstract

DNA extraction is a very important step for DNA genetic analysis in peach palm (Bactris gasipaes Kunth.). The aim of this work was to develop a methodology of DNA extraction. Fresh, frozen and dried leaves were utilized. DNA from fresh leaves were purified one time while frozen and dried leaves was purified two times. DNA bands were coloured intensively showing a high amount of purified DNA and with an excellent resolution in agarosis gel 2%, such for fresh leaves as much as for frozen and dried leaves.

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References

GAIOTTO, F. A. Influência sobre herança quantitativa e estrutura genética em populações naturais de Euterpe edulis MART. utilizando marcadores microsatelites. Piracicaba: ESALQ, 2001. 122 p.
SOUSA, N. R. de; RODRIGUES, D. P.; CLEMENT, C. R.; NAGAO, E. O.; ASTOLFI-FILHO, S. Discriminação de raças primitivas de pupunha (Bactris gasipaes) na Amazônia brasileira por meio de marcadores moleculares (RAPDs). Acta Amazônica, v. 3, n. 4, p. 539-545, 2001.
SAWAZAKI, H. E.; BOVI, M. L. A.; SODEK, L.; COLOMBO, C. A. Diversidade genética em palmeiras através de isoenzimas e RAPD. Revista Brasileira de Biologia, v. 58, n. 4, p. 681-691, 1998.

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Published

2003-12-30

How to Cite

HAMZE, Ahmad Abdul Latif; KALIL FILHO, Antonio Nascim; SOUSA, Valderês Aparecida de; SANTOS, Álvaro Figueredo dos; NEVES, Edinelson José Maciel. DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations. Pesquisa Florestal Brasileira, [S. l.], n. 47, p. 117–122, 2003. Disponível em: https://pfb.sede.embrapa.br/pfb/article/view/1612. Acesso em: 25 apr. 2026.

Issue

No. 47 (2003): jul./dez.

Section

Scientific Notes

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