Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin

Aline Meneguzzi; Enéas Ricardo Konzen; Marcio Carlos Navroski; Samila Silva Camargo; Mariane de Oliveira Pereira; Léo Rufato; Queli Cristina Lovatel

doi:10.4336/2019.pfb.39e201701550

Authors

Aline Meneguzzi Universidade do Estado de Santa Catarina, Departamento de Engenharia Florestal https://orcid.org/0000-0003-0652-8888
Enéas Ricardo Konzen Universidade do Estado de Santa Catarina, Departamento de Engenharia Florestal https://orcid.org/0000-0001-5176-7410
Marcio Carlos Navroski Universidade do Estado de Santa Catarina, Departamento de Agronomia https://orcid.org/0000-0002-2645-9763
Samila Silva Camargo Universidade do Estado de Santa Catarina, Departamento de Engenharia Florestal https://orcid.org/0000-0002-0659-1361
Mariane de Oliveira Pereira Universidade Federal do Paraná, Departamento de Engenharia Florestal https://orcid.org/0000-0001-5518-7464
Léo Rufato Universidade do Estado de Santa Catarina, Departamento de Agronomia https://orcid.org/0000-0001-9545-7035
Queli Cristina Lovatel Universidade do Estado de Santa Catarina, Departamento de Engenharia Florestal https://orcid.org/0000-0002-0120-9119

DOI:

https://doi.org/10.4336/2019.pfb.39e201701550

Keywords:

Micropropagation, Cytokinins, Callogenesis

Abstract

Sequoia sempervirens (D. Don) Endl. is a conifer which produces high-quality wood with potential industrial applications. However, the species shows low germination rates. This study was devoted to optimizing a protocol for shoot multiplication of S. sempervirens through micropropagation. Two genotypes, adapted to southern Brazil, constituted the source of explants for consecutive experiments aiming in vitro multiplication. We used the traditional MS (Murashige & Skoog) culture medium at 50% of its original concentration, supplemented with plant growth regulators under two approaches. For multiplication we combined Î±-naphtalene acetic acid (NAA) and 6-benzilaminopurine (BAP) at distinct concentrations. In the second experiment, we tested concentrations of three cytokinins types (BAP, kinetin and 2-isopentheiladenine ). NAA at 0.1 mg L^-1 enabled the production of shoots with higher mean length for the main branch. The use of kinetin in low concentrations provided the best performance for shoot multiplication, differing between two genotypes. We outline a recommendation of the most suitable plant growth regulators and their concentration for shoot multiplication of S. sempervirens, which might assist further work aiming at adventitious rooting and acclimatization.

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