In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa

Authors

  • Aline Moritz Pontíficia Universidade Católica do Paraná-PUC
  • Juliana Degenhardt Embrapa Florestas
  • Leonardo Ferreira Dutra Embrapa Clima Temperado
  • Fabrício Augusto Hansel Embrapa Florestas
  • Bruno Henrique de Lima Pontifícia Universidade Católica do Paraná - PUC-PR
  • Cristina do Rosário Batista Franceschi Pontifícia Universidade Católica do Paraná - PUC-PR
  • Luziane Franciscon Pontifícia Universidade Católica do Paraná - PUC-PR

Keywords:

BAP, activated charcoal, sucrose, conservation.

Abstract

Several species of the Lauraceae family are endangered, due to the high value of their woods,
and the consequent exploitation of natural populations. Among them, Ocotea porosa, O. odorifera and O.
catharinensis, native from South Brazil, have recalcitrant seeds, what makes even more dificult their natural
regeneration. This work aimed to evaluate in vitro seed germination and multiplication of O. odorifera and O.
catharinensis in culture medium supplemented with activated charcoal and/or BAP and in vitro seed germination
and multiplication of O. porosa in culture medium supplemented with several concentrations of sucrose. The
effect of BAP and activated charcoal were tested in the multiplication of O. catharinensis and O. odorifera and
the effect of sucrose concentration (30, 60, 90 ou 120 g.L-1) was tested in the multiplication of O. porosa. The
germination percentage was higher than 85% for all species. BAP promoted the highest multiplication rates for
O. odorifera. O. porosa showed the best results for in vitro germination and multiplication on 60 g.L-1 sucrose.
The establishment of O. catharinensis was achieved, but after some weeks, in all media tested, the explants
showed abnormalities, and finally died.

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Published

2010-02-26

How to Cite

MORITZ, Aline; DEGENHARDT, Juliana; DUTRA, Leonardo Ferreira; HANSEL, Fabrício Augusto; LIMA, Bruno Henrique de; FRANCESCHI, Cristina do Rosário Batista; FRANCISCON, Luziane. In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa. Pesquisa Florestal Brasileira, [S. l.], n. 59, p. 37, 2010. Disponível em: https://pfb.sede.embrapa.br/pfb/article/view/14. Acesso em: 25 apr. 2026.

Issue

No. 59 (2009): jul./dez.

Section

Articles

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