Germinação in vitro de sementes e multiplicação de Calophyllum brasiliense

Sheila Susy Silveira; Juliana Degenhardt-Goldbach; Marguerite Germaine Ghislaine Quoirin

doi:10.4336/2016.pfb.36.87.1168

Authors

Sheila Susy Silveira Universidade Federal do Paraná https://orcid.org/0000-0002-1793-6847
Juliana Degenhardt-Goldbach Embrapa Florestas https://orcid.org/0000-0001-9463-2380
Marguerite Germaine Ghislaine Quoirin Universidade Federal do Paraná https://orcid.org/0000-0003-3190-6895

DOI:

https://doi.org/10.4336/2016.pfb.36.87.1168

Keywords:

Gibberellic acid, Benzylaminopurine, Germination speed index

Abstract

Calophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination was reduced with fungicide added to culture medium (23 to 6.4%), although bacterial contamination increased (24 to 36%). In another experiment, seeds were immersed in plant preservative mixture (PPMâ„¢) prior to surface disinfestation. By combining immersion for 48 h and 2 mL L-1 in culture medium, contamination was only 6%. Seeds immersion in GA3 prior to surface disinfestation reduced root formation as concentration increased. Germination rate and GSI were reduced, respectively, from 72% and 0.129 (24 h) to 60% and 0.092 (48 h) according to exposure time to GA3. After 90 days in multiplication medium containing benzylaminopurine, average number of shoots per nodal segment was 3.4. In conclusion, in vitro germination of C. brasiliense seeds is feasible in sucrose-free WPM medium and reaches a high contamination-free rate (up to 93.3%).

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Author Biographies

Sheila Susy Silveira, Universidade Federal do Paraná

http://lattes.cnpq.br/0370363410002238

Juliana Degenhardt-Goldbach, Embrapa Florestas

http://lattes.cnpq.br/3956913701572749

Marguerite Germaine Ghislaine Quoirin, Universidade Federal do Paraná

http://lattes.cnpq.br/7947529909503630

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