Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen
DOI:
https://doi.org/10.4336/2017.pfb.37.92.1145Keywords:
Genipa americana, Germplasm conservation, Seed preservationAbstract
The objective of this study was to evaluate the effects of two cryoprotectants followed by thawing on the physiological potential of Genipa americana L. seeds. Two experiments testing 12 treatments were conducted, one for each cryoprotectant, both in a factorial scheme of 6 × 2 (cryoprotectant concentrations × thawing methods). We tested 0, 5, 10, 15, 20, and 25% for dimethyl sulfoxide, and 0, 0.25, 0.5, 0.75, 1.0, and 1.25 M for sucrose. The thawing methods were fast (38 °C for 30 min) and slow (25 °C for 4 h). The seeds were immersed in the cryoprotectant solution for 3 h, stored for 120 h, and then thawed. The seeds were then sown in substrate (sand and vermiculite, 1:1). Emergence percentage along with speed index, length, fresh and dry matter mass of seedlings were evaluated. Dimethyl sulfoxide and sucrose can be used as cryoprotectants in G. americana seeds. Thawing should be slow when treating seeds with dimethyl sulfoxide.Downloads
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References
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Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Regras para análise de sementes. Brasília, DF, 2009. 395 p.
Coelho, N. et al. Germination and cryopreservation tolerance of seeds from the rare aromatic species Thymus lotocephalus. Scientia Horticulturae, v. 145, n. 20, p. 84-86, 2012. DOI: 10.1016/j.scienta.2012.07.031.
Engelmann, F. In vitro conservation of tropical plant germplasm: a review. Euphytica, v. 57, n. 3, p. 227-243, 1991.
Farias, D. C. et al. Qualidade fisiológica de sementes de jatobá submetidas a diferentes temperaturas criogênicas. Revista Brasileira de Produtos Agroindustriais, v. 8, n. 1, p. 67-74, 2006.
Fonseca, A. G. et al. Qualidade fisiológica de sementes Pinus elliottii Engelm submetidas e diferentes métodos de armazenamento. Cerne, v. 18, n. 3, p. 457-463, 2012. DOI: 10.1590/S0104-77602012000300013.
Gallo, C. M. Cultivo in vitro e criopreservação de Vasconcellea quercifolia A. St. Hil. 2015. 104 f. Tese (Doutorado em Agronomia) - Universidade Federal de Lavras, Lavras.
Goldfarb, M. et al. Armazenamento criogênico de sementes de pinhão manso (Jatropha curcas L.) Euphorbiaceae. Biotemas, v. 23, n. 1, p. 27-33, 2010. DOI: 10.5007/2175-7925.2010v23n1p27.
Kholina, A. B. & Voronkova, N. M. Conserving the gene pool of far Eastern plants by means of seed cryopreservation. Biology Bulletin, v. 35, n. 3, p. 262-269, 2008. DOI: 10.1134/S1062359008030060.
Kholina, A. B. & Voronkova, N. M. Seed cryopreservation of some medicinal legumes. Journal of Botany, v. 2012. Article 186891, 2012. DOI: 10.1155/2012/186891.
Li, D. & Pritchard, H. W. The science and economics of ex situ plant conservation. Trends in Plant Science, v. 14, n. 11, p. 614-621, 2009. DOI: 10.1016/j.tplants.2009.09.005.
Maguire, J. D. Speeds of germination-aid selection and evaluation for seedling emergence and vigor. Crop Science, v. 2, n. 1, p. 176-177, 1962.
Molina, T. F. et al. Crioconservação em sementes de cebola. Revista Brasileira de Sementes, v. 28, n. 3, p. 72-81, 2006. DOI: 10.1590/S0101-31222006000300011.
Motta, L. B. et al. Cryostorage of sunflower seeds. Bioscience Journal, v. 30, n. 2, p. 312-319, 2014.
Padro, M. D. A. et al. Cryopreservation of white mulberry (Morus alba L.) by encapsulation-dehydration and vitrification. Plant Cell, Tissue and Organ Culture, v. 108, n. 1, p. 167-172, 2012. DOI: 10.1007/s11240-011-0017-5.
Pence, V. C. The possibilities and challenges of in vitro methods for plant conservation. Kew Bulletin, v. 65, n. 4, p. 539-547, 2010.
Pita, J. M. et al. Seed cryopreservation of seven Spanish native pine species. Silvae Genetica, v. 47, n. 4, p. 220-223, 1998.
R Core Team. R: A language and environment for statistical computing. Viena: R Foundation for Statistical Computing, 2012.
Rocha, M. S. et al. Crioconservação de sementes de algodão. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 13, n. 2, p. 312-318, 2009. DOI: 10.1590/S1415-43662009000300013.
Sakai, A. Cryopreservation of germplasm of woody plants. In: Bajaj, Y. P. S. (Ed.). Biotechnology in agriculture and forestry. v. 32. Cryopreservation of plant germplasm I. Berlin: Springer-Verlag, 1995. p. 53-69.
Tao, D. & Li, P. H. Classification of plan cell cryoprotectants. Journal of Theoretical Biology, v. 123, n. 1, p. 305-310, 1986. DOI: 10.1016/S0022-5193(86)80245-4.
Tresena, L. T. et al. Qualidade fisiológica da semente de ipê rosa (Tabebuia hepptahylla (Vellozo) Toledo) submetidas à crioconservação. Revista Brasileira de Produtos Agroindustriais, v. 11, n. 1, p. 87-92, 2009. DOI: 10.1590/S0104-77602012000300013.
Vasanth, K. & Vivier, M. A. Improved cryopreservation procedure for long term storage of synchronized culture of grapevine. Biologia Plantarum, v. 55, n. 2, p. 365-369, 2011.
Voronkova, N. M. & Kholina, A. B. Conservation of endemic species from the Russian far east using seed cryopreservation. Biology Bulletin, v. 37, n. 5, p. 496-501, 2010. DOI: 10.1134/S1062359010050092.
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Published
2017-12-29
How to Cite
SOBRINHO, Severino de Paiva; ALBUQUERQUE, Maria Cristina de Figueiredo e; LUZ, Petterson Baptista da; NEVES, Leonarda Grillo; CAMILI, Elisangela Clarete. Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen. Pesquisa Florestal Brasileira, [S. l.], v. 37, n. 92, p. 421–428, 2017. DOI: 10.4336/2017.pfb.37.92.1145. Disponível em: https://pfb.sede.embrapa.br/pfb/article/view/1145. Acesso em: 19 apr. 2025.
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