Metodologia de extração de DNA para análise genética de populações de pupunha (Bactris gasipaes Kunth.)

Ahmad Abdul Latif Hamze; Antonio Nascim Kalil Filho; Valderês Aparecida de Sousa; Ãlvaro Figueredo dos Santos; Edinelson José Maciel Neves

DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations

Authors

Ahmad Abdul Latif Hamze Unesp
Antonio Nascim Kalil Filho Embrapa Florestas
Valderês Aparecida de Sousa Embrapa Florestas
Ãlvaro Figueredo dos Santos Embrapa Florestas
Edinelson José Maciel Neves Embrapa Florestas

Keywords:

Heart-palm, Arecaceae, Protocol

Abstract

DNA extraction is a very important step for DNA genetic analysis in peach palm (Bactris gasipaes Kunth.). The aim of this work was to develop a methodology of DNA extraction. Fresh, frozen and dried leaves were utilized. DNA from fresh leaves were purified one time while frozen and dried leaves was purified two times. DNA bands were coloured intensively showing a high amount of purified DNA and with an excellent resolution in agarosis gel 2%, such for fresh leaves as much as for frozen and dried leaves.

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Published

2003-12-30

Issue

No. 47 (2003): jul./dez.

Section

Scientific Notes

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How to Cite

HAMZE, A. A. L. et al. DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations. Pesquisa Florestal Brasileira, n. 47, p. 117–122, 30 Dec.2003.

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DNA extraction methodology for genetic analysis of peach palm (Bactris gasipaes Kunth.) populations

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