Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
DOI:
https://doi.org/10.4336/2015.pfb.35.84.798Keywords:
Natural dyes, Soil microbiology, Euterpe oleraceaAbstract
Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.
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Copyright (c) 2015 Aline Lourdes Martins Silva, Marcos Diones Ferreira Santana, John César de Jesus Pereira, Milena Pupo Raimam, Ulisses Brigatto Albino
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