Desinfestação e introdução in vitro de segmentos nodais de Acacia mearnsii

Ecléia Alexandra Poltronieri Buda Salles; Giovana Bomfim Alcantara; Marguerite Germaine Ghislaine Quoirin; Antonio Natal Gonçalves; Antonio Rioyei Higa

doi:10.4336/2017.pfb.37.92.1392

Disinfection and in vitro establishment of Acacia mearnsii nodal segments

Authors

Ecléia Alexandra Poltronieri Buda Salles Federal University of Paraná
Giovana Bomfim Alcantara Federal University of Paraná http://orcid.org/0000-0001-5578-5843
Marguerite Germaine Ghislaine Quoirin Federal University of Paraná
Antonio Natal Gonçalves Universidade de São Paulo - Esalq
Antonio Rioyei Higa Federal University of Paraná

DOI:

https://doi.org/10.4336/2017.pfb.37.92.1392

Keywords:

Black wattle, Clones, Cutting

Abstract

Due to low rates of rooting it is difficult to propagate selected clones of Acacia mearnsii by cuttings. The purpose of this study was to define a protocol for disinfection of nodal segments for in vitro introduction, aiming to improve the species genetically for forest plantation. Combination of different products concentration (70% ethyl alcohol, sodium hypochlorite, plant preservative mixture and mercuric chloride), immersion period and size of nodal segment were tested. The best disinfection treatment was the combination of 70% ethanol, 0.4% mercuric chloride, sodium hypochlorite at 2% in nodal segments of 10 mm, with 80% survival.

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Published

2017-12-29

Issue

Vol. 37 No. 92 (2017): out./dez.

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How to Cite

SALLES, E. A. P. B. et al. Disinfection and in vitro establishment of Acacia mearnsii nodal segments. Pesquisa Florestal Brasileira, v. 37, n. 92, p. 485–491, 29 Dec.2017.

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Disinfection and in vitro establishment of Acacia mearnsii nodal segments

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